منابع مشابه
Unit 27.2 Identification of RNA Binding Proteins by UV Crosslinking
One of the major focuses of modern biology is to understand the dynamics of RNAprotein interactions, including factors that interact with mRNA, rRNA, tRNA, snRNA, hnRNA, siRNA, and viral RNA. Identification the direct interactions between proteins and RNA has greatly advanced our knowledge about the function of RNA-protein machines. UV crosslinking is a straightforward and powerful tool in this...
متن کاملIdentification of RNA-protein contacts within functional ribonucleoprotein complexes by RNA site-specific labeling and UV crosslinking.
A variety of cellular processes are carried out by highly complex ribonucleoprotein (RNP) particles in which multiple RNA-RNA, RNA-protein, and protein-protein interactions occur. The spliceosome, which executes the nuclear pre-mRNA splicing reaction, is a particularly striking example of a complex RNP, containing a minimum of 50 distinct protein components as well as five small nuclear RNAs. I...
متن کاملEmerging Techniques: Uv Laser Footprinting and Protein-dna Crosslinking
Protein-nucleic acids interactions are of key importance in gene regulation. The laser strategy for studying these interactions is based on the rapid "freezing" of the processes through specific, single pulse induced irreversible photoreactions. Two different approaches, either proteinDNA crosslinking or footprinting, may be used, depending on the object to be studied. Protein-DNA crosslinking ...
متن کاملNonradioactive UV cross-linking assay for the study of protein-RNA binding.
100 μL 2% (wt/vol) 5-bromo-4-chloro3-indolyl-β-D-galactopyranoside (Xgal) and 20 μL 100 mM isopropyl β-Dthiogalactopyranoside (IPTG). Individual blue colonies were selected and grown overnight in Superbroth containing 50 μg/μL ampicillin. Plasmid DNA was isolated using the PERFECTprep Kit (5 Prime→3 Prime, Boulder, CO, USA), and the construct was checked by restriction digestion and sequencing...
متن کاملEvaluation of corneal symmetry after UV corneal crosslinking for keratoconus
Purpose The purpose of this study was to assess UV corneal crosslinking (CXL) treatment outcomes for keratoconus by evaluating the corneal regularity in patients through follow-up using the Oculus Pentacam. Patients and methods A total of 18 eyes from CXL patients with keratoconus were studied before and after CXL treatment, and six eyes from six patients who were not treated with CXL served ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2017
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.2193